Frequently Asked Questions
Find answers to common questions about peptide dosing, reconstitution, the calculator, and everything else about Peptide Simplified.
Getting Started
What is Peptide Simplified?
Peptide Simplified is a free educational tool that helps researchers calculate precise peptide reconstitution volumes and injection dosages. Our calculator covers 65+ peptides with dosing protocols, storage guidelines, vendor comparisons, and educational guides.
Is this calculator free to use?
Yes, the peptide calculator is completely free. We also offer optional features like account creation (to save your peptide stacks) and a downloadable spreadsheet pack for $1 that includes reference charts for all peptides.
Do I need to create an account?
No account is required to use the calculator, browse peptide profiles, read guides, or compare vendor prices. Creating a free account lets you save your peptide stacks for easy reference later.
What peptides does the calculator support?
We support 65+ peptides across categories including healing and recovery (BPC-157, TB-500), growth hormone secretagogues (Ipamorelin, CJC-1295), GLP-1 weight management (Semaglutide, Tirzepatide), nootropics (Selank, Semax), and many more.
Using the Calculator
How do I use the dosage calculator?
Select your peptide from the search bar, enter your vial size in milligrams, and input your desired dose. The calculator automatically determines the optimal reconstitution volume and tells you exactly how many units to draw on your insulin syringe.
What does 'units to inject' mean?
Units refer to the markings on an insulin syringe. One full 1ml insulin syringe equals 100 units. Our calculator converts your desired dose (in mcg or mg) into the number of units to draw on your syringe based on how much water you added to your peptide vial.
How is the reconstitution volume calculated?
Each peptide has an optimal reconstitution ratio based on common dosing ranges. We calculate the ideal amount of bacteriostatic water to add so that your doses fall within a practical range on an insulin syringe.
What is the difference between mcg and mg dosing?
Micrograms (mcg) and milligrams (mg) are both units of weight. 1 mg equals 1,000 mcg. Most peptides are dosed in micrograms, but some like TB-500 and GLP-1 agonists use milligrams. Our calculator lets you toggle between both units.
How does weight-based dosing work?
Some peptides are dosed based on body weight (e.g., 5 mcg per kg). When you enter your weight in the optional field, the calculator adjusts the recommended dose according to established weight-based protocols for that specific peptide.
Reconstitution & Storage
What is reconstitution?
Reconstitution is the process of adding a liquid solvent (typically bacteriostatic water) to the freeze-dried peptide powder in a vial to create an injectable solution. This must be done carefully to avoid damaging the peptide molecules.
Why do I need bacteriostatic water instead of regular sterile water?
Bacteriostatic water contains 0.9% benzyl alcohol, which prevents bacterial growth in the vial over multiple uses. Regular sterile water has no preservative, meaning the solution can grow bacteria within 24 hours of being pierced.
Why should I never shake a peptide vial?
Shaking creates bubbles and physical forces that can break apart (denature) the delicate peptide molecules, reducing their effectiveness. Always swirl the vial gently or let it sit until the powder dissolves naturally.
How long do reconstituted peptides last?
Most reconstituted peptides last 3-4 weeks when properly refrigerated at 2-8 degrees Celsius. Some peptides like semaglutide can last 6-8 weeks, while others like MOTS-c should be used within 7-10 days.
Can I freeze reconstituted peptides?
No. Freezing a reconstituted peptide can cause the solution to expand and potentially crack the peptide molecules, destroying their structure. Unreconstituted (powder) peptides can be stored frozen for long-term storage.
Injection Technique
What type of syringe should I use?
Insulin syringes with 29-31 gauge needles are standard for subcutaneous peptide injection. They come in 0.3ml (30 units), 0.5ml (50 units), and 1ml (100 units) sizes.
Where should I inject peptides?
The most common subcutaneous injection sites are the stomach area (avoiding 2 inches around the navel), the front or outer thigh, and the back of the upper arm. For healing peptides like BPC-157, injecting near the injury site may provide more targeted effects.
Why is site rotation important?
Repeatedly injecting in the same spot can cause lipohypertrophy (fatty lumps under the skin) and may reduce absorption over time. Rotating between at least 3-4 sites helps prevent these issues.
About the Site
Is Peptide Simplified medical advice?
No. Peptide Simplified is strictly for educational and research purposes. The information provided should not be considered medical advice. Peptides are research chemicals not approved for human use by the FDA. Always consult a qualified healthcare provider.
Where does your dosing information come from?
Our dosing protocols are compiled from published research literature, FDA label information where available, established community protocols from peptide research forums, and clinical trial data.
How often is the content updated?
We regularly update peptide information, vendor pricing, and educational content. New peptides, guides, and features are added based on community feedback and emerging research.